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Microbial contamination of buildings and building material

Using of biotechnologies in building industry and environment protection, using of secondary raw materials


Technopark Kralupy in collaboration with The University of Chemistry and Technology, Prague pursues in fields of microbiology, biotechnology, environmental technology and using of wastes as a secondary raw materials following activities:


  • Isolation and determination of bacterial and fungal contamination of external masonry (plasters), internal spaces (walls - wood, masonry, decorating), floors (various floorings – linoleum, wood, various types of floating floors etc., ceilings, cellars etc.)





Microscope, equipped with fluorescent attachment and digital camera


  • Isolation and determination of presence of degradeting microflora and her biodiversity in a soil contaminate by organic pollutants on building sites
  • Isolation and determination of microbial contamination in the air of contaminated buildings - rooms
  • Determination of microbial purity of water sources (water wells, running and stagnant water etc.)


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Laminar boxes

  • Biodecontamination of soils, waste, ground and surface waters contaminated by organic pollutants (oil hydrocarbons – petrol, disel oil, mineral oil, dissolving agents and aromatic hydrocarbons cokechemical origin)


At this activity it is used the biodegradable effect of blend of microorganismgenus Acinetobacter and Klebsiella, usually in a demobilized form in a flow bioreactor.


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Inner filling of flow bioreactor



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Purification course of ground water contamitated by kerosine using flow bioreactor


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Purification of waste water using flow bioreactor


Bioremediation technology gain sense by a need to decontamitate interal premises of halls, courtyards and historically preservated objects, where is very limited possibility of removing of pollutants from floors, soil, subsoil etc. Typical example is the bioremediation of brick floor of  riding hall on the Lednice castle.


  • Using of  mycorrhiza in combination with bio coal on planting and growing of plants when afforesting and optimalizing of urbanism


Application of mycorrhizal fungi in a combination with bio coal, supplemented by a fertilizers and hydrocolloids is attractive especially by growing forest woody plants on bare spaces, places used for pooring of material of mining, in areas with deficiency of soil moisture, microflora and nutriens.


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Cultures of ektomycorrhizal fungi on GKCH agar



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Planting material inokulated by ektomymycorrhizal fungius


Application of mycorrhizal fungi with bio coal brings following advantages:

- increased lenght of roots

- enlarged surface for receipt of mineral nutriens,

- possibility of exploitation bigger volume of soil,

- increased intake of mineral nutrients and some microelements,

- selective absorption of some ions from a soil,

- better possibility use of very low concentrations of nutriens in a soil,

- better use of some inaccessible nutriens forms,

- improved resistance against invasion of root pests and parasites,

- improved tolerance against toxins,

- improved tolerance against low temperatures and stressful influence of dryness

- improved tolerance to changes of pH 

- radical improving of quality and fertility of soil via improvement of water regime,

- improving of cations exchange capacity (CEC),

- reduction of quantity applied artificial fertilizers,

- reduction of flush out of nutrients,

- increasing of available elements Ca, Mg, P and K in soil,

- reduction of content carbon dioxine and next greenhouse gases in air,

- reduction of emissions of nitrous oxide and methane,

- better structure of soil and ability to retain moisture,

- support growth of mycorrhizal fungi include vesicular-arbuscular mycorrhiza,

- increasing of microbial biomass in the soil and soil microbial respiration,

- increasing of soil aggregation in consequence of increase fungal hyphae,

- significant reduction of risk of occurrence of plants diseases 

a significant reduction in the risk of occurrence of plant diseases,

- support symbiotic nitrogen fixation in legumes plants.

  • Surface protection of building materials prior to the occurrence and development of mold in the interior and exterior


Using catalysts photoproducts

To prepare the catalysts photoproducts was worked out in which it is used as a starting material waste from titanium engineering production of medical devices and special resistant materials based on titanium.

 Obtained photolytic catalysts are used not only in removing the mold, but also for the disposal of organic pollutants contained in the exhaust gases of internal combustion engines, in cigarette smoke in the flue exhibiting undesirable odor of the product range of technologies like.

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Efficacy photolytic catalyst rhodamine B


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 Inhibitory effect on growth of photolytic catalyst Aspergillus niger

Using "smart sponge" Pythium oligandrum

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Pythium oligandrum


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Cultivation of 'smart sponges' Pythium oligandrum on a solid substrate

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 Zoospore Pythium oligandrum

  • Processing technology and utilization of waste rubber



Group of Mikrobial contamination
Technopark Kralupy VŠCHT Praha
Žižkova 7, 278 01 Kralupy nad Vltavou
Tel. 220 446 106, 776 805 452


: 14.12.2016 18:56, : Karel Hrušovský

Technopark Kralupy
Žižkova 7
Kralupy nad Vltavou
278 01
© 2017 Technopark Kralupy